DIRECT ELISA

 

DIRECT ELISA :

DIRECT ELISA The direct ELISA uses the method of directly labeling the antibody itself. Microwell plates are coated with a sample containing the target antigen, and the binding of labeled antibody is quantitated by a colorimetric, chemiluminescent, or fluorescent end-point.

DIRECT ELISA :

DIRECT ELISA Advantages of Direct Detection Quick methodology since only one antibody is used. Cross-reactivity of secondary antibody is eliminated. Disadvantages of Direct Detection Immunoreactivity of the primary antibody may be reduced as a result of labeling. Labeling of every primary antibody is time-consuming and expensive. No flexibility in choice of primary antibody label from one experiment to another. Little signal amplification.